Journal article
Probing Endosomal Escape Using pHlexi Nanoparticles
N Kongkatigumjorn, C Cortez-Jugo, E Czuba, ASM Wong, RY Hodgetts, APR Johnston, GK Such
Macromolecular Bioscience | WILEY-V C H VERLAG GMBH | Published : 2017
Abstract
The effective escape of nanocarriers from endosomal compartments of the cell remains a major hurdle in nanomedicine. The endosomal escape of pH-responsive, self-assembled, dual component particles based on poly[2-(diethylamino)ethyl methacrylate)(PDEAEMA) and poly(ethylene glycol)-b-poly[2-(diethylamino)ethyl methacrylate) (PEG-b-PDEAEMA) has been recently reported. Herein, we report that polymer molecular weight (Mn) can be used to tune endosomal escape of nanoparticle delivery systems. PDEAEMA of Mn 7 kDa, 27 kDa, 56 kDa and 106 kDa was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization and co-assembled with PEG-b-PDEAEMA (16 kDa) via nanoprecipitation. ..
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Awarded by Australian Research Council through the Future Fellowship Scheme
Funding Acknowledgements
This work was supported by the Australian Research Council through the Future Fellowship Scheme (FT120100564 to G.K.S. and FT110100265 to A.P.R.J.) and Centre of Excellence in Convergent Bio-Nano Science and Technology (A.P.R.J.). A.P.R.J. was also supported through the Monash University Larkin's Fellowship Scheme. The authors thank Dr. E. Hanssen (Melbourne Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute) for cryo-EM imaging. G. Neode (Monash Institute of Pharmaceutical Sciences, Monash University) is acknowledged for assistance with the fluorescence plate reader.